You need laparoscopic equipment, technical expertise and only a limited number of inseminations can be carried out on each sheep. Normally 150 x 106 sperm/sequins are frozen. Double the amount of sperm would be advantageous for vaginal insemination. The aim of this study is to find out whether deep freezing with twice the sperm concentration (300 x 106 sperm / semen) has a negative influence on the thawed semen. Fluorescence microscopic examinations of the acrosome, Kovacs staining of the sperm and the measurement of motility parameters are used.
Link to press release: Frozen sheep sperm
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Link to the final report: On the semen quality of frozen sheep semen: comparison of two sperm concentrations